MOLECULAR DEVICES R8128 Flipr Membrane Potential Evaluation Assay Kit 206829
( Brand: Molecular Devices ), ( Manufacturer Part Number: R8128 ), ( Model: FLIPR ), ( Type: Membrane Potential Evaluation Assay Kit ), ( Country/region Of Manufacture: United States )
Review MOLECULAR DEVICES Flipr Membrane Potential Evaluation Assay Kit
The R8128 Flipr Membrane Potential Evaluation Assay Kit, manufactured by Molecular Devices with the product code 206829, is an essential tool for researchers studying cellular ionic homeostasis and membrane potential changes. This kit offers a reliable and highly sensitive method for measuring changes in membrane potential in a variety of cell types.
The R8128 Flipr Assay Kit is based on the principle of fluorescence resonance energy transfer (FRET). The assay employs a genetically encoded FRET biosensor, such as the FliprMembrane or FliprFluo4 N1, which is introduced into the cells of interest. When the membrane potential changes, the biosensor undergoes a conformational change, resulting in a change in FRET efficiency. This change in FRET efficiency is then measured using a fluorescence microscope or plate reader, providing a quantitative readout of membrane potential changes.
The R8128 Flipr Assay Kit includes a buffer solution that is optimized for the Flipr biosensors, as well as a positive control solution. The kit also includes detailed protocols for preparing the cell suspension, loading the biosensor into the cells, and measuring the FRET signal. The protocols are designed to be user-friendly and can be easily adapted to a variety of cell types and experimental conditions.
One of the key advantages of the R8128 Flipr Assay Kit is its high sensitivity and specificity. The biosensors used in the assay are highly selective for changes in membrane potential and are not affected by other cellular processes such as calcium ion signaling. The assay is also highly sensitive, with a detection limit of 1-2 mV.
Another advantage of the R8128 Flipr Assay Kit is its versatility. The assay can be used to study a wide range of cellular processes that are associated with changes in membrane potential, including ion channel activity, membrane potential-dependent signaling, and cellular responses to drugs and toxins. The assay can also be used to study membrane potential changes in live cells, providing real-time information about cellular processes.
In summary, the R8128 Flipr Membrane Potential Evaluation Assay Kit is a powerful tool for researchers studying cellular ionic homeostasis and membrane potential changes. Its high sensitivity and specificity, as well as its versatility and user-friendly protocols, make it an ideal choice for a wide range of experimental applications. Whether you are studying ion channel activity, membrane potential-dependent signaling, or cellular responses to drugs and toxins, the R8128 Flipr Assay Kit can help you gain insights into the underlying mechanisms of these processes and reveal new therapeutic targets.
Pros of buying a R8128 Flipr Membrane Potential Evaluation Assay Kit (Molecular Devices, Cat# 206829):1. High-throughput: This kit allows for the simultaneous measurement of membrane potential changes in up to 96 wells, making it an efficient tool for large-scale experiments.
2. Reliable and reproducible results: The Flipr Membrane Potential Assay Kit uses fluorescence-based technology, which provides accurate and consistent results, reducing the likelihood of experimental errors.
3. User-friendly: The kit includes easy-to-follow instructions and comes with pre-calibrated Flipr membrane potential dye, making it easy for researchers without extensive experience in membrane potential measurements to use.
4. Versatile: The R8128 Flipr Membrane Potential Evaluation Assay Kit can be used with various cell types and applications, including ion channel studies, cellular toxicity, and drug discovery.
Cons of buying a R8128 Flipr Membrane Potential Evaluation Assay Kit (Molecular Devices, Cat# 206829):1. Cost: The kit can be expensive, especially for researchers with limited budgets.
2. Limited cell types: While the kit can be used with various cell types, it may not be suitable for certain cell lines or primary cells that may require different experimental conditions.
3. Requires specialized equipment: The R8128 Flipr Membrane Potential Evaluation Assay Kit requires the use of a Fluorescence Microplate Reader, which can be an additional investment for researchers who do not already have one.
Conclusion:The R8128 Flipr Membrane Potential Evaluation Assay Kit (Molecular Devices, Cat# 206829) is a reliable and efficient tool for measuring membrane potential changes in up to 96 wells. Its user-friendly design and versatility make it an attractive option for researchers in the fields of ion channel studies, cellular toxicity, and drug discovery. However, its high cost and limited compatibility with certain cell types and experimental conditions should be considered before making a purchase.
Recommendation:If the R8128 Flipr Membrane Potential Evaluation Assay Kit fits within your research budget and experimental requirements, it is a worthwhile investment for its accuracy, high-throughput capabilities, and versatility. However, it is recommended to first evaluate whether the kit's performance is suitable for your specific cell type and experimental design before making a purchase. Additionally, considering alternative, less expensive methods for membrane potential measurement may be beneficial for researchers with limited resources.
Details:
No return accepted.
All return shipping charges must be prepaid by the customer.
specifications analyticalinstrumentzo:
- brand: Molecular Devices
- mpn: R8128
- model: Flipr
- type: Membrane Potential Evaluation Assay Kit
- country/region of manufacture: United States
Shipping analyticalinstrumentzo:
- handling: 3 days
- type: Calculated
Payments analyticalinstrumentzo:
- PayPal
Returns analyticalinstrumentzo:
- type: Money Back
- details: Restocking fee: No
- policy: returns accepted
- paid by: Buyer
- time: 14 Days
General analyticalinstrumentzo:
- condition: New
- business industrial > healthcare life science lab equipment business industrial > analytical instruments lab equipment
- in stock: Yes
Ratings:
- AAA Items works perfectly, Will do business again. 10-12-17
- As described. great seller! 10-05-17
- Beckman microplate carriers came in good condition, balanced pair, spin well. 10-03-17
- awesome!!! 10-06-17
- AAA Items works perfectly, Will do business again. 10-15-17
Offer analyticalinstrumentzo:
- quantity: 1
- best offer: false
- Started: October 16, 2017, 11:00 pm
- Sold: 0
- bids: 0
Seller analyticalinstrumentzo:
- city: Moreno Valley, California
- zipcode: 92557
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