
( Brand: Phenomenex ), ( Type: Hplc Column )
The Phenomenex 5 m Aqua 125A 250mm C18 1x is a high-quality, ultra-pure, and ultra-performance liquid chromatography (LC) column designed for use in analytical and preparative separations. This column is specifically engineered with a 3.0mm inner diameter and a 250mm length, providing a generous capacity for sample loading and separation.
The column is packed with 5 m C18 particles, which are highly porous and offer excellent chromatographic retention and separation efficiency. The C18 stationary phase is made of a chemically bonded, trifluoroacetic acid (TFA)-compatible, and stable material, ensuring excellent column stability and repeatability in a wide range of pH conditions.
The 125A silica-based particle material used in this column is highly uniform and offers excellent chemical resistance, making it suitable for use with a wide range of mobile phases, including organic solvents and aqueous buffers. The particle material is also highly cross-linked, providing excellent mechanical strength and minimizing particle-to-particle variability, ensuring consistent and reproducible results.
The Aqua 125A 250mm C18 1x column is designed with a 1 m layer thickness, providing excellent mass transfer efficiency, faster analysis times, and higher throughput, making it an excellent choice for high-pressure, high-speed separations. This column is also compatible with a wide range of LC instruments, including HPLC, UHPLC, and UPLC systems.
In summary, the Phenomenex 5 m Aqua 125A 250mm C18 1x is a high-performance LC column that delivers excellent separation efficiency, reproducibility, and stability, making it an excellent choice for a wide range of analytical and preparative separations. Its large inner diameter, highly porous C18 particles, and high-quality silica-based material make it an excellent choice for high-pressure, high-speed separations, and its compatibility with a wide range of LC instruments makes it a versatile and versatile tool in any LC laboratory.
Pros of buying a Phenomenex 5um Aqua 125A 250mm C18 1x:1. High-quality material: Phenomenex is a reputable manufacturer known for producing high-quality columns. This particular column is made of Aqua C18, a type of silica-based material that is highly efficient and stable, making it suitable for a wide range of applications.
2. Excellent separation efficiency: The 3.0mm particle size of the stationary phase provides a large surface area for retention, resulting in excellent separation efficiency. This is particularly beneficial for complex mixtures with closely eluting compounds.
3. Durable and long-lasting: The 250mm length of the column allows for increased capacity and longer run times, making it a cost-effective choice in the long run. Additionally, the column is designed to withstand harsh conditions, ensuring its longevity.
Cons of buying a Phenomenex 5um Aqua 125A 250mm C18 1x:1. High initial investment: The price of this column may be a significant barrier for some users, especially for those with limited budgets.
2. Requires specialized equipment: To make the most of this column's performance, you will need specialized HPLC equipment, which can be expensive and may require maintenance and upkeep.
Conclusion:The Phenomenex 5um Aqua 125A 250mm C18 1x is a high-quality HPLC column with excellent separation efficiency, durability, and long-lasting performance. While the initial investment may be high, the column's ability to handle complex mixtures and its long-term cost-effectiveness make it a worthwhile investment for many users. If you have the necessary HPLC equipment and the budget to accommodate the high price tag, this column is an excellent choice for separating and analyzing a wide range of compounds.
Recommendation:If you require a high-performance HPLC column for separating and analyzing complex mixtures, the Phenomenex 5um Aqua 125A 250mm C18 1x is an excellent choice. However, make sure to consider your budget and equipment capabilities before making a purchase. If the cost is prohibitive or if you have less demanding separation needs, you may want to consider alternative column options with lower particle sizes, shorter lengths, or different stationary phases to reduce the overall cost.
Used 1x only everything in photos is included.
This column was bought new and tested for a complex organic acid mix and since it did not work it was capped and has been stored since.