( Brand: Phenomenex Biosep Sec-s 4000 ), ( Manufacturer Part Number: 00H-2147-K0 )
The 00h-2147-K0 LC 300 4000 column from Biosep, in collaboration with Phenomenex, is a top-tier chromatography column designed for high-performance liquid chromatography (HPLC) applications. This column features a 7.8 mm inner diameter and a length of 4000 mm, making it suitable for handling large sample volumes and providing excellent separation efficiency.
The column is packed with SEC-S stationary phase, a highly cross-linked, hydrophilic, and chemically bonded silica material. This stationary phase provides excellent column stability and a wide linear range of operation, making it ideal for separating proteins, peptides, and other large biomolecules. The 5 m particle size of the stationary phase provides excellent efficiency and reduces analysis time, while the 300 pore size ensures that even the largest biomolecules can be separated with ease.
The 00h-2147-K0 LC 300 4000 column is designed to operate at temperatures ranging from 4 to 80 C, providing flexibility in terms of the types of analytes that can be separated. The column is also compatible with a wide range of mobile phases, including aqueous buffers, organic solvents, and mixed mobile phases.
In summary, the 00h-2147-K0 LC 300 4000 column is a high-performance chromatography column that provides excellent separation efficiency, stability, and compatibility with a wide range of analytes and mobile phases. Its large inner diameter and length make it suitable for handling large sample volumes, while its SEC-S stationary phase provides excellent separation of large biomolecules. This column is an excellent choice for HPLC applications in the biopharmaceutical industry and other research fields.
The (00h-2147-K0 LC 300 4000 column, 7.8 mm ID, Biosep 500, Phenomenex SEC-S) is a high-performance liquid chromatography (HPLC) column designed for size exclusion chromatography (SEC) applications. Here are some pros and cons of buying this column:
Pros:1. High-quality materials: The column is made of high-quality materials, including a stainless steel body and a Biosep 500 end-capping material, which ensures durability and long-term use.
2. Large pore size: The 7.8 mm ID and 4000 pore size of the column allow for the separation of large biomolecules, such as proteins and viruses, with high resolution and efficiency.
3. Excellent separation efficiency: The SEC-S stationary phase used in the column provides excellent separation efficiency, which results in sharp peaks and high resolution.
4. Wide range of applications: The column is suitable for a wide range of SEC applications, including protein purification, size-exclusion-based fractionation, and biomolecule characterization.
Cons:1. High cost: The cost of the column is relatively high compared to other HPLC columns, which may make it less affordable for some researchers.
2. Limited compatibility: The column may not be compatible with all HPLC systems, and users should check the compatibility of the column with their specific HPLC system before purchasing.
3. Limited column length: The column is only 300 mm long, which may limit the separation capacity for some applications, especially for large sample volumes.
In conclusion, the (00h-2147-K0 LC 300 4000 column, 7.8 mm ID, Biosep 500, Phenomenex SEC-S) is a high-quality, high-performance SEC column suitable for a wide range of applications. Its large pore size and excellent separation efficiency make it an excellent choice for separating large biomolecules. However, its high cost and limited compatibility and column length may be a drawback for some researchers. Therefore, before making a purchase, it is essential to consider the specific requirements of the application and the availability of compatible HPLC systems. Based on the pros and cons, it is recommended to consider the column as an investment for high-quality, high-performance size exclusion chromatography applications.
All glassware equipment should be carefully and properly cleaned before use.