
( Manufacturer Part Number: 17-0547-01 ), ( Type: Pharmacia Biotech ), ( Country/region Of Manufacture: Sweden )
The 17-0547-01 FPLC Biotech Protein Pharmacia Mono Column HPLC is a high-performance liquid chromatography (HPLC) column designed for the purification of proteins and large biomolecules. This column is part of the FPLC (Fast Protein Liquid Chromatography) system, a versatile tool for protein separation and purification.
The Mono Column HPLC features a monolithic structure, which means that the stationary phase is integrated into the column matrix itself. This design offers several advantages over traditional packed-bed columns, such as increased efficiency, higher flow rates, and lower backpressure. The monolithic structure also allows for easier column conditioning and regeneration, reducing downtime and maintenance requirements.
The 17-0547-01 FPLC Biotech Protein Pharmacia Mono Column HPLC is specifically designed for protein purification and has a wide pH range (2-10) and a high temperature stability (up to 80 C). The column matrix is made of porous glass, which provides excellent chemical resistance and durability. The column has a total volume of 50 mL and a nominal bed volume of 40 mL, making it suitable for handling large sample volumes and high protein loads.
The Mono Column HPLC is compatible with various elution buffers and can be used with a wide range of detection methods, including UV, refractive index, and fluorescence. The column is also compatible with various FPLC systems, such as the AKTA purifier and AKTA pure, making it a versatile tool for protein purification in a laboratory setting.
In summary, the 17-0547-01 FPLC Biotech Protein Pharmacia Mono Column HPLC is a high-performance and versatile tool for protein purification. Its monolithic structure, wide pH and temperature range, and high chemical resistance make it a reliable and efficient option for biotech and pharmaceutical applications.
Pros of buying 17-0547-01 FPLC Biotech Protein Pharmacia Mono Column HPLC:1. High-performance liquid chromatography (HPLC) is a powerful technique for separating, identifying, and quantifying compounds in complex mixtures. This column is specifically designed for protein purification, making it an excellent choice for biotech applications.
2. The FPLC system is known for its robustness and reliability, ensuring consistent results over time.
3. The mono-column design allows for high capacity and resolution, reducing the need for multiple column setups and saving time and resources.
4. The column is manufactured by Pharmacia, a reputable company in the field of HPLC, ensuring high-quality materials and manufacturing processes.
Cons of buying 17-0547-01 FPLC Biotech Protein Pharmacia Mono Column HPLC:1. The cost of the column and the FPLC system can be high, making it a significant investment for some laboratories or researchers.
2. The operation and maintenance of the FPLC system require a certain level of expertise, which may not be available in all laboratories.
3. The system may require regular calibration and cleaning to maintain its performance, which can be time-consuming and costly.
Conclusion:The 17-0547-01 FPLC Biotech Protein Pharmacia Mono Column HPLC is a high-quality, high-performance tool for protein purification in biotech applications. While the cost and expertise required for operation and maintenance are potential drawbacks, the benefits of its robustness, high capacity, and resolution make it a valuable investment for laboratories and researchers in the field.
Recommendation:If the cost and expertise requirements are within your budget and capabilities, the 17-0547-01 FPLC Biotech Protein Pharmacia Mono Column HPLC is an excellent choice for protein purification in biotech applications. If the cost is a concern, consider alternative HPLC systems or methods with lower upfront costs, although they may not offer the same level of performance or versatility.
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