
( Brand: Ge ), ( Manufacturer Part Number: 17-1165-01 ), ( Country Of Manufacture: United States ), ( Modified Item: No )
The 17-1165-01 Ge Hiprep 16-60 Sephacryl Preparative Column from Pharmacia is a high-quality, versatile tool designed for the separation and purification of proteins, nucleic acids, and other macromolecules in the laboratory setting. This column is part of the Ge Healthcare's Sephacryl family, renowned for its superior separation efficiency and excellent resolution.
The Ge Hiprep 16-60 Sephacryl Preparative Column is a pre-packed column, measuring 16 cm in height and 60 mm in diameter. It is filled with Sephacryl S-300 HR resin, a highly cross-linked and rigid gel, which provides excellent separation capacity and high flow rates. The resin is made from a dextran matrix, making it gentle on biological samples and suitable for a wide range of applications.
The column comes with a built-in sample loop, allowing for easy and reproducible sample loading. It also features a bottom outlet for gradient elution and a top outlet for sample injection. The column has a total volume of 411 ml, with a bed volume of 365 ml, providing ample space for large sample loads.
The Ge Hiprep 16-60 Sephacryl Preparative Column is designed for use with a wide range of solvents, including buffers and salts, making it suitable for a variety of separation methods. It is compatible with common HPLC systems, allowing for easy integration into existing workflows.
In summary, the 17-1165-01 Ge Hiprep 16-60 Sephacryl Preparative Column from Pharmacia is a powerful and versatile tool for the separation and purification of macromolecules. Its high capacity, excellent separation efficiency, and compatibility with a wide range of solvents make it a valuable asset in any laboratory focused on protein and nucleic acid research.
Pros of buying a 17-1165-01 Ge HIPrep 16-60 SEPakryl Preparative Column from Pharmacia:1. High capacity: This column has a large volume of 60 ml, which makes it suitable for handling large sample sizes.
2. High efficiency: The SEPakryl material used in the column provides excellent separation efficiency and resolution, which is crucial for obtaining pure compounds.
3. Wide pH range: The column can be used over a wide pH range of 2-12, making it versatile for use with a variety of sample types.
4. Long lifespan: The column is designed to provide consistent performance over an extended period, reducing the need for frequent replacements.
Cons of buying a 17-1165-01 Ge HIPrep 16-60 SEPakryl Preparative Column from Pharmacia:1. High cost: The column is relatively expensive compared to other preparative columns available in the market.
2. Limited compatibility: The column is specifically designed for use with aqueous solutions and may not be suitable for use with organic solvents.
3. Requires specialized equipment: The column requires specialized equipment such as a pump, a fraction collector, and a UV-VIS detector to operate, which can be expensive to acquire.
Conclusion:The 17-1165-01 Ge HIPrep 16-60 SEPakryl Preparative Column from Pharmacia is a high-performance preparative column that provides excellent separation efficiency, high capacity, and a wide pH range. However, its high cost and limited compatibility may make it a less suitable option for some researchers. If you have the budget and require a high-performance preparative column, this product is an excellent choice.
Recommendation:If you are looking for a preparative column with excellent separation efficiency, high capacity, and a wide pH range, the 17-1165-01 Ge HIPrep 16-60 SEPakryl Preparative Column from Pharmacia is a great option. However, if you have a limited budget or require a column that can be used with organic solvents, you may want to consider other options available in the market.
New in sealed box old stock pharmacia hiprep sephacryl s-100 preparative column cat no, lot .