
( Brand: Thermo Dionex ), ( Manufacturer Part Number: 059135 )
The 2.1 x 100 mm Thermo Dionex ACQUITY UPLC BEH C18 Reversed-Phase LC Column 5 m (Part No. 059135) is a high-performance, analytical-scale, reversed-phase column designed for use with liquid chromatography (LC) systems. This column is manufactured by Thermo Dionex, a reputable brand known for its quality and reliability in the field of chromatography.
The ACQUITY UPLC BEH C18 column is a 2.1 mm x 100 mm, which makes it ideal for use in micro-LC applications, where space and sample consumption are critical. The column features a 5 m particle size, which provides excellent efficiency and resolution, allowing for the separation of complex mixtures. The column is packed with 120 pore size C8 stationary phase, providing excellent retention and separation properties for a wide range of analytes, including organic and inorganic compounds.
The column is designed with a reversed-phase mechanism, which means that the stationary phase is hydrophobic and the mobile phase is hydrophilic. This type of chromatography is commonly used for the separation of polar and non-polar compounds, such as peptides, proteins, and small molecules. The reversed-phase mechanism allows for the efficient separation of analytes based on their hydrophobicity, providing high-resolution chromatograms.
The ACQUITY UPLC BEH C18 column is specifically designed for use with Thermo Dionex's UPLC systems, but it can also be used with other LC systems that are compatible with C18 stationary phases and reversed-phase chromatography. The column is made of high-quality materials, including a stainless-steel body and end fittings, ensuring durability and long-term performance.
In summary, the 2.1 x 100 mm Thermo Dionex ACQUITY UPLC BEH C18 Reversed-Phase LC Column 5 m (Part No. 059135) is a high-performance, micro-LC column that provides excellent efficiency and resolution for the separation of complex mixtures. Its reversed-phase mechanism, 5 m particle size, and C8 stationary phase make it a versatile and reliable option for a wide range of LC applications.
Pros of buying a 2.1x100mm Thermo Dionex 059135 Acclaim 120 C8 Reversed-Phase LC Column 5um:1. High-quality material: The column is made of high-quality C8 material, which is well-known for its ability to retain and separate analytes in liquid chromatography.
2. Small size: The 2.1mm inner diameter of the column makes it suitable for use in small-scale and micro-scale applications, such as UHPLC (Ultra-High Performance Liquid Chromatography).
3. Excellent reproducibility: The column is designed with a high degree of uniformity, ensuring excellent reproducibility of chromatographic separations.
4. Long lifespan: The column is made with durable materials, ensuring a long lifespan and reducing the need for frequent replacements.
5. Compatibility: The column is compatible with various LC systems, making it a versatile choice for various applications.
Cons of buying a 2.1x100mm Thermo Dionex 059135 Acclaim 120 C8 Reversed-Phase LC Column 5um:1. High price: The column is relatively expensive, which may make it less accessible to some researchers and laboratories.
2. Requires specialized equipment: The column requires specialized LC equipment to operate, which may not be readily available to all laboratories.
3. Limited capacity: The small size of the column limits its capacity for analyte loading, which may affect the sensitivity of the analysis.
4. Limited resolution: The column may not provide the same level of resolution as larger-diameter columns, which may limit its applicability for certain separations.
In conclusion, the 2.1x100mm Thermo Dionex 059135 Acclaim 120 C8 Reversed-Phase LC Column 5um is a high-quality, durable, and versatile option for small-scale and micro-scale LC applications. However, its high price and limited capacity may make it less suitable for some researchers and laboratories. If the benefits of the column outweigh its drawbacks, I would recommend considering it as a potential purchase. If budget is a concern, there may be other options with similar performance at a lower price point.
1x100mmThermo Dionex acclaim 120 c8 high performance reversed-phase lc column n: 059135total carbon: c18 c8pore diameter: dimensions: 1 x 100mm particle size: 5 inventory 5d84630. New Thermo Dionex acclaim 120 c8 reversed-phase lc column 5um 2.