
( Brand: Phenomenex ), ( Manufacturer Part Number: 00G-3034-E0 )
The Phenomenex Waters PrimeSphere C18 HC 250 x 4.6 mm HPLC Column, with the item code 00g-3034-e0, is a high-performance liquid chromatography (HPLC) column designed for separating and analyzing complex mixtures of organic compounds.
This column is part of the PrimeSphere series, which is known for its excellent separation efficiency and high resolution. The C18 (C8-C18) stationary phase is made of highly porous, spherical particles, which provides excellent mass transfer and improved peak shape. The HC (High Capacity) version of this column has a larger particle size of 2.5 m, which allows for higher loading capacity and faster separations.
The column is 250 mm long and 4.6 mm in internal diameter, providing a sufficient separation length and a good balance between efficiency and sample capacity. The 4.6 mm diameter is also a common size for HPLC systems, making it easy to integrate into existing chromatography setups.
The Phenomenex Waters PrimeSphere C18 HC 250 x 4.6 mm HPLC Column is made of fused silica, which is highly resistant to chemical and thermal degradation, ensuring long-term stability and reliability. The column is also equipped with a stainless steel guard column to protect the analytical column from contamination.
This column is suitable for a wide range of applications, including environmental analysis, pharmaceutical and biotech research, and quality control. The Phenomenex Waters PrimeSphere C18 HC 250 x 4.6 mm HPLC Column is a reliable and high-performance tool for separating and analyzing complex mixtures in HPLC.
Pros of buying Phenomenex Waters PrimeSphere C18 HC 250 x 4.6mm HPLC Column (00G-3034-E0):1. High efficiency: The column's sub-2 micron particle size and optimized pore size provide high efficiency and fast separations.
2. Robustness: The column's proprietary particle technology ensures excellent durability and reproducibility.
3. High loading capacity: The column can handle high sample loads, which can reduce analysis time and improve throughput.
4. Versatile: The C18 phase is suitable for a wide range of analytes and can be used in various applications such as peptide mapping, protein purification, and small molecule analysis.
5. Compatible with various mobile phases: The column is compatible with a wide range of mobile phases, including acids, bases, and organic solvents.
Cons of buying Phenomenex Waters PrimeSphere C18 HC 250 x 4.6mm HPLC Column (00G-3034-E0):1. High cost: The column is relatively expensive compared to some other HPLC columns in the market.
2. Requires specialized equipment: The column requires specialized HPLC equipment, which can be expensive to purchase and maintain.
3. Limited capacity: The column's 250mm length may not be suitable for larger sample volumes or complex separations that require longer columns.
4. Limited compatibility with certain mobile phases: While the column is compatible with a wide range of mobile phases, some specific mobile phases may not be suitable for use with this column.
Conclusion:The Phenomenex Waters PrimeSphere C18 HC 250 x 4.6mm HPLC Column (00G-3034-E0) is a high-performance column that offers excellent efficiency, robustness, and high loading capacity. However, its high cost and limited compatibility with certain mobile phases may be a drawback for some users. Ultimately, the decision to purchase this column depends on the specific requirements of the application and the available budget.
Recommendation:If you require a high-performance column for peptide mapping, protein purification, or small molecule analysis, the Phenomenex Waters PrimeSphere C18 HC 250 x 4.6mm HPLC Column (00G-3034-E0) is a recommended option due to its excellent efficiency, robustness, and high loading capacity. However, if cost is a concern, you may want to consider alternative options with similar performance characteristics but at a lower price point.
Please look at the pics for details. Very good used condition.
Sold as is in a pictured described condition. 6mm 5 micron hplc column 557 with original box. You are buying on one Phenomenex primesphere 5 c18 hc 250 x 4.