
( Brand: Phenomenex ), ( Manufacturer Part Number: 00B-4251-B0 )
The Phenomenon Jupiter 4U Proteo 90A HPLC Column, model 00b-4251-b0, is a high-performance liquid chromatography (HPLC) column designed for protein and peptide separations. This column is part of Phenomenex's Jupiter Proteo series, which is known for its exceptional performance in proteomic applications.
The 00b-4251-b0 column features a 50 mm length, 1.00 mm internal diameter, and a 90 pore size. This combination of dimensions allows for efficient separation of proteins and peptides with a wide range of molecular weights and charges. The column is packed with Phenomenex's proprietary HyperSil Gold silica material, which provides excellent retention and high sensitivity, enabling the detection of low-abundance peptides and proteins.
The column surface is further modified with a unique bonded phase consisting of a polymer-based amide ligand, which provides excellent protein and peptide binding capacity and retention. This ligand is highly selective for basic and acidic amino acids, making it an ideal choice for large-scale protein and peptide separations.
The 00b-4251-b0 column is also designed to be highly robust and stable, with a rugged construction that can withstand high pressures and harsh solvents. This makes it suitable for use in a wide range of HPLC systems, including those with automated sample preparation and gradient elution capabilities.
In summary, the Phenomenon Jupiter 4U Proteo 90A HPLC Column, model 00b-4251-b0, is a high-performance tool for protein and peptide separations. Its unique bonded phase, rugged construction, and high-capacity ligand make it an ideal choice for researchers and analysts working in proteomics, mass spectrometry, and other related fields.
For those looking for a replacement or an upgrade for their current HPLC column, the 00b-4396-a0 Phenomenex Proteo 90A HPLC Column, also available, is a compatible and high-quality alternative. This column features the same 50 mm x 1.00 mm dimensions and 90 pore size as the 00b-4251-b0, but with a slightly different bonded phase that may offer slightly different separation properties. Both columns are designed to provide excellent performance and long-term stability in HPLC applications.
Pros of buying a Phenomenex Jupiter 4U Proteo 90A HPLC Column (00B-4251-B0):1. High efficiency: The column has a high number of theoretical plates (90A), which results in better separation efficiency and resolution of complex mixtures.
2. Wide pH range: The column is designed for a wide pH range (1.8-8.0), making it suitable for a variety of protein and peptide separations.
3. Large inner diameter: The column has a large inner diameter (2.1 mm), which allows for higher sample load and faster analysis times.
4. Durable materials: The column is made of high-quality materials, such as fused-silica and endcapping materials, which ensures long-term stability and durability.
Cons of buying a Phenomenex Jupiter 4U Proteo 90A HPLC Column (00B-4251-B0):1. High cost: The column is relatively expensive, which may be a limiting factor for some budget-conscious researchers.
2. Requires specific solvents and buffers: The column is optimized for use with specific solvents and buffers, which may require additional investment in consumables.
3. Limited compatibility with some instrumentation: The column may not be compatible with all HPLC systems, which may limit its use for some researchers.
Conclusion:The Phenomenex Jupiter 4U Proteo 90A HPLC Column (00B-4251-B0) is a high-quality, high-performance column that is well-suited for the separation of complex protein and peptide mixtures. Its high efficiency, wide pH range, and large inner diameter make it a versatile and powerful tool for protein and peptide analysis. However, its high cost and limited compatibility with some instrumentation may be a limiting factor for some researchers. If the benefits of the column outweigh the costs for your specific research needs, then it is a worthwhile investment.
Recommendation:If you are looking for a high-performance HPLC column for protein and peptide analysis, the Phenomenex Jupiter 4U Proteo 90A HPLC Column (00B-4251-B0) is a strong contender. Its high efficiency, wide pH range, and large inner diameter make it a versatile and powerful tool for separating complex mixtures. However, it is important to consider the cost and compatibility issues before making a purchase decision. We recommend doing your research and consulting with your instrument manufacturer to ensure that the column is compatible with your specific HPLC system.
Hplc column Phenomenex jupiter 4u proteo 90a 50 x1. The pictures are of actual items being sold. 00mm 4 micron P/N 00B-4396-A0 this listing is for one Phenomenex jupiter 4u proteo 90a 50 x1.